Journal: Reproductive Sciences
Article Title: Rosiglitazone-Mediated Activation of PPARγ Induces PlGF Expression in Trophoblast Cells
doi: 10.1007/s43032-025-01868-w
Figure Lengend Snippet: Rosiglitazone induces nuclear translocation and down-regulation of PPARγ. JEG-3 cells exposed to 21% O 2 or 1.5% O 2 (with reoxygenation at 21% O 2 ) conditions in the presence of 10 (R10) or 50 (R50) µM rosiglitazone. ( a ) Western blot analyses of PPARγ levels normalized to β-actin by one-way ANOVA with post hoc tests to determine significant differences between groups; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 ( n = 3). ( b ) Representative confocal microscopy images of PPARγ localization (green) under basal conditions and in the presence of 50 µM rosiglitazone. DAPI staining is shown in red and nuclear colocalization in yellow; bar = 50 μm
Article Snippet: Protein levels were normalized to a housekeeping protein, β‐actin (1:5000; Cell Signaling Technology).
Techniques: Translocation Assay, Western Blot, Confocal Microscopy, Staining